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![Western blot analysis of lysates from wild type (WT) and Gasdermin E (N terminal) knockout (KO) HeLa cells using [KO Validated] Cleaved Gasdermin E (N terminal) Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and GSDME knockout (KO) HeLa cells were treated with TNF-α (50ng/mL) and CHX (10μg/mL) at 37℃ for 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.|WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC70388-01_WB_05_83-232.jpg)
CATALOG NUMBER: 83-232
CATALOG NUMBER: 83-914
![Western blot analysis of lysates from Rat brain using [KO Validated] CTIP1/BCL11A Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC1486_WB_02_84-339.jpg)
CATALOG NUMBER: 84-339
![Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0474_WB_02_81-322.jpg)
CATALOG NUMBER: 81-322
![Western blot analysis of lysates from wild type (WT) and DNMT3A knockout (KO) 293T cells using [KO Validated] DNMT3A at 1:500 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0138_KO-WB_01_81-564.jpg)
CATALOG NUMBER: 81-564
CATALOG NUMBER: 81-939
![Western blot analysis of various lysates using [KO Validated] FGFR2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0480_WB_01_81-334.jpg)
CATALOG NUMBER: 81-334
![Western blot analysis of various lysates using [KO Validated] Flotillin 1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC76972-01_WB_01_83-630.jpg)
CATALOG NUMBER: 83-630
![Western blot analysis of extracts from wild type (WT) and FOXN2 knockout (KO) HeLa(KO) cells, using [KO Validated] FOXN2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC58450-01_KO-WB_01_82-328.jpg)
CATALOG NUMBER: 82-328
![Western blot analysis of lysates from wild type (WT) and FTO knockout (KO) 293T(KO) cells, using [KO Validated] FTO Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC52115-01_KO-WB_01_82-277.jpg)
CATALOG NUMBER: 82-277
