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![Western blot analysis of various lysates using [KO Validated] ACLY Rabbit mAb at 1:2000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20 s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC56109-01_WB_01_83-378.jpg)
![Western blot analysis of lysates from wild type (WT) and ACLY knockout (KO) 293T cells using [KO Validated] ACLY Rabbit mAb at 1:10000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20 s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0281-01_WB_01_83-756.jpg)
