The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1).TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
A sandwich ELISA was performed using anti-TIGIT mouse monoclonal antibodies to capture the TIGIT extracellular domain antigen and anti-TIGIT single domain antibodies for detection.
| Target | Cat.no. | Capture | Detection | Host | Datasheet |
|---|---|---|---|---|---|
| TIM-3 Antibody Pair | RFP-1001 | RF16104 | RF16103-Biotin | Mouse - Mouse |  |
ProSci’s monoclonal immune checkpoint antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, these TIM-3 antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. They are guaranteed to work for their tested applications.
Sets consist of 20µg aliquots of several mouse monoclonal antibodies with different binding features allowing for selection of the best clone for your application.
ProSci’s Risk-Free TIM-3 antibodies are rigorously tested and guaranteed to work for their designated applications. TIM-3 antibodies were raised against the extracellular domain of human TIM-3.
KEY:
| Cat. No. | Product Type | Source |
|---|---|---|
| RF16100-01 | Recombinant Protein | HEK293 |
| Cat. No. | Name | Host | Clonality | Applications |
|---|---|---|---|---|
| 8659 | TIM-3 Antibody | Rabbit | Polyclonal | ELISA, IF, IHC-P, WB |
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