SARS-CoV-2 Envelope and Membrane Reagents

Upon the entry of SARS-CoV-2 into host cells, from the full-length genomic RNA (29,903 nt) that serves as an mRNA, the first set of viral proteins are translated from the long (>21 kb) open reading frame ORF1ab (1).

The ORF1ab-encoded polyprotein is subsequently processed into 16 individual non-structural proteins (NSP) by two proteases PLpro/nsp3 and 3CLpro/nsp5. The 3’ half of ORF1ab, ORF1b, encodes a variety of enzymes critical for viral transcription and replication (1). In addition to the genomic RNA, nine major sub-genomic RNAs are produced (1). ORF1a and ORF1b are also separately translated and, as in all coronaviruses, translation of ORF1b in SARS-CoV-2 requires a programmed -1 ribosomal frameshift (-1 PRF) (2).

The genome RNA is packaged by the structural proteins to assemble progeny virions. The shorter sub-genomic RNAs encode conserved structural proteins spike protein (S), an envelope protein (E), membrane protein (M) and nucleocapsid protein (N), and several accessory proteins. SARS-CoV-2 is known to have at least 6 accessory proteins (ORFs 3a, 7, 7a, 7b, 8, and 10)

ProSci Incorporated offers antibodies and recombinant proteins representing most of the SARS-CoV-2 proteome. Below is a list of SARS-CoV-2 envelope and membrane reagents to serve as tools for your research needs!