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![Western blot analysis of lysates from HT-1080 cells using [KO Validated] Bax Rabbit mAb at 1:10000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC5006-06-02_WB_01_81-298.jpg)
![Western blot analysis of various lysates, using [KO Validated] Beclin 1 Rabbit mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC52858-01_WB_01_82-216.jpg)
![Western blot analysis of various lysates using [KO Validated] Calreticulin Mouse mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/AMC50035-01_WB_01_83-467.jpg)
![Western blot analysis of various lysates using [KO Validated] Caspase-8 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0028_WB_01_81-483.jpg)
![Western blot analysis of various lysates, using [KO Validated] CDK5 Rabbit mAb at1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC53344-01_WB_01_82-647.jpg)
![Western blot analysis of various lysates using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73214-01_WB_01_83-590.jpg)
![Western blot analysis of lysates from wild type (WT) and CDKN2A/p16INK4a knockout (KO) 293T cells using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0661_WB_02_81-201.jpg)
![Western blot analysis of various lysates using [KO Validated] cIAP1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0168_WB_01_81-580.jpg)
![Western blot analysis of lysates from wild type (WT) and GSK3α knockout (KO) HeLa cells, using [KO Validated] GSK3α Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0462_KO-WB_01_81-341.jpg)