DB

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of various lysates, using Acetyl-Histone H4-K12 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, ChIP-seq, DB, ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Western blot analysis of various lysates using Acetyl-Histone H4-K16 Rabbit mAb at 1:20000 dilution incubated overnight at 4℃. HeLa cells, NIH/3T3 cells and C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, DB, ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Western blot analysis of various lysates using Acetyl-Histone H4-K5 Rabbit mAb at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Confocal imaging of HeLa cells (treated with TSA) and HeLa cells (untreated) cells using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, DB, ELISA, IF/ICC
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA
Conjugate:
Unconjugated
Species Reactivity:
Independent species
List Size:
100 uL
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA
Conjugate:
Unconjugated
Species Reactivity:
Independent species
List Size:
100 uL
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA
Conjugate:
Unconjugated
Species Reactivity:
Independent species
List Size:
100 uL
The Anti-Digoxigenin/Digoxin Rabbit mAb are tested in Dot Blot against digoxin labelled oligonucleotide(Dig-1,Dig-2 and Dig-3) and unlabelled oligonucleotide(Dig-4) Dig-1 :5'AGCTAAC/iDigdT/ACTAGCT(Biotin)3' Dig-2 :5'(Digoxin)AGCTAACTACTAGCT(Biotin)3' Dig-3 :5'(Biotin)AGCTAACTACTAGCT(Digoxin)3' Dig-4 :5'AGCTAACTACTAGCT(Biotin)3'
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of various lysates, using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb at1:4000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with PADI4 using Citrulline-Histone H3-R8 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours. 293F cells transfected with PADI4 were treated by CaCl2 (10 mM) and Ionomycin (10 μM) at 37℃ for 4 hours after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 5s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Other (Wide Range Predicted)
List Size:
100 uL