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![Western blot analysis of lysates from wild type (WT) and BRD2 knockdown (KD) 293T cells using [KD Validated] BRD2 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC56992-01_WB_02_82-493.jpg)
![Western blot analysis of lysates from THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb at 1:72000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC57989-03_WB_01_82-855.jpg)
