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![Western blot analysis of lysates from CACO-2 cells using [KD Validated] NOX1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC72716-01_WB_01_83-570.jpg)
![Western blot analysis of various lysates, using [KD Validated] ZNF143 Rabbit mAb at 1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC62979-01_WB_01_83-024.jpg)
![Western blot analysis of various lysates using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73214-01_WB_01_83-590.jpg)
![Western blot analysis of lysates from wild type (WT) and CDKN1B/p27KIP1 knockdown (KD) HeLa cells using [KO Validated] CDKN1B/p27KIP1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0455_WB_01_81-366.jpg)
![Western blot analysis of lysates from Jurkat cells, using [KO Validated] MLH1 Mouse mAb at 1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/AMC0417-1_WB_01_81-995.jpg)
![Western blot analysis of lysates from wild type (WT) and MLH1 knockout (KO) HeLa cells using [KO Validated] MLH1 Rabbit mAb at 1:8000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC53543-01_WB_01_83-130.jpg)
![Western blot analysis of lysates from wild type(WT) and MSH2 knockout (KO) HeLa cells, using [KO Validated] MSH2 Rabbit mAb at1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC55799-01_KO-WB_01_82-122.jpg)
![Western blot analysis of various lysates, using [KO Validated] MSH6 Rabbit mAb at 1:3000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC57727-01_WB_01_83-129.jpg)
![Western blot analysis of lysates from 293T cells using [KO Validated] p53 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC2618_WB_01_82-637.jpg)