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![Western blot analysis of lysates from wild type (WT) and Rbm7 knockout (KO) mouse testis using [KO Validated] Rbm7 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.|WB samples for antibody validation are kindly provided by Dr. Shaorong Gao.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73985-01_WB_02_83-542.jpg)
