FC

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from A549 cells using ICAM-1/CD54 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. A549 cells were treated with TNF-α (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from Rat lung using ICAM-1/CD54 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and CHO cells transfected with CD278 using CD278 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Flow cytometry: 1X10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface-stained with IL-27RA/WSX-1 Rabbit mAb or PE Rabbit IgG isotype control (5 μl/Test,blue line), followed by PE Donkey anti-rabbit Antibody staining. Non-fluorescently stained cells were used as blank control (red line).
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using IL-33R/ST2 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from Jurkat cells, using IL2 Rabbit mAb at 1:1000 dilution. Jurkat cells were treated with TPA (40 nM),A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Flow cytometry: 1X10^6 293F cells (negative control,left) and 293F (Transfection,right) cells were surface-stained with IL23R Rabbit mAb or Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Integrin alpha 3/CD49c Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and A549 cells (right) were surface-stained with Integrin β5/ITGB5 Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
FC
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with KIR2DL2, using KIR2DL2/KIR2DL3 Rabbit mAb at 1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL