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Immunoglobulins

Categories

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Flow cytometry: 1X10^6 293T-CD90.1 (Transfection,left) cells and 293T-CD90.2 (Transfection,right) cells were surface-stained with CD90.2 Rabbit mAb or Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

CD90.2 Rabbit Recombinant Antibody (83-204)

CATALOG NUMBER: 83-204

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of various lysates using CD90/Thy1 Rabbit mAb at 1:42000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

CD90/Thy1 Rabbit Recombinant Antibody (83-601)

CATALOG NUMBER: 83-601

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Mouse IgD (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgD (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgD (Fc) has highly specific recognition of Mouse IgD while no or minimal cross reactivity to Mouse IgG1、Mouse IgG2a、Mouse IgG2b、Mouse IgG2c、Mouse IgG3、Mouse IgM、Mouse IgE、Mouse IgA、Rat IgG、Human IgG.

HRP conjugated Rabbit Anti-Mouse IgD (Fc) Recombinant Antibody (84-796)

CATALOG NUMBER: 84-796

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgG2b (Fc) has highly specific recognition of Mouse IgG2b while no or minimal cross reactivity to Mouse IgG1、Mouse IgG2a、Mouse IgG2c、Mouse IgG3、Mouse IgM、Mouse IgE、Mouse IgD、Rat IgG、Human IgG.

HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) Recombinant Antibody (84-795)

CATALOG NUMBER: 84-795

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of various lysates, using Human IgA1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Human IgA1 Rabbit Recombinant Antibody (82-674)

CATALOG NUMBER: 82-674

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Human IgD Rabbit mAb at 1:3000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

Human IgD Rabbit Recombinant Antibody (82-438)

CATALOG NUMBER: 82-438

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of recombinant IGHG3 protein, using Human IgG3 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Human IgG3 Rabbit Recombinant Antibody (82-783)

CATALOG NUMBER: 82-783

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from Human serum, using Human Kappa Light Chain Rabbit mAb at 1:900 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 0.5s.

Human Kappa Light Chain Rabbit Recombinant Antibody (82-711)

CATALOG NUMBER: 82-711

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IF-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates, using Human Kappa Light Chain Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): HAP1|Exposure time: 60s.

Human Kappa Light Chain Rabbit Recombinant Antibody (82-981)

CATALOG NUMBER: 82-981

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from Mouse spleen using Mouse IgM Rabbit mAb at 1:3000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Mouse IgM Rabbit Recombinant Antibody (83-111)

CATALOG NUMBER: 83-111

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL