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![Western blot analysis of lysates from wild type (WT) and MEK2 knockout (KO) HeLa cells, using [KO Validated] MEK2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0361_KO-WB_01_81-353.jpg)
![Western blot analysis of lysates from Mouse brain using [KO Validated] MEK7 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0712_WB_03_81-251.jpg)
![Western blot analysis of lysates from wild type (WT) and MyD88 knockout (KO) HeLa cells, using [KO Validated] MyD88 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0350_KO-WB_01_81-356.jpg)
![Western blot analysis of various lysates using [KO Validated] P Glycoprotein Rabbit mAb at 1:3000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): MCF7|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0470_WB_01_81-365.jpg)
![Western blot analysis of various lysates using [KO Validated] PTEN Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC2643_WB_01_81-373.jpg)
![Western blot analysis of various lysates using [KO Validated] Smad1 Rabbit mAb at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC52106-01_WB_01_82-194.jpg)
![Western blot analysis of lysates from wild type (WT) and Smad1 knockout (KO) HeLa cells, using [KO Validated] Smad1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0365_KO-WB_01_82-640.jpg)
![Western blot analysis of lysates from wild type (WT) and Src knockout (KO) HeLa cells, using [KO Validated] Src Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0378_KO-WB_01_81-385.jpg)
![Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0046_KO-WB_01_81-495.jpg)