![Western blot analysis of extracts from wild type(WT) and NRF2 knockout (KO) HeLa cells, using [KO Validated] NRF2 Rabbit mAb (A3577) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0806_KO-WB_01_83-713.jpg)
CATALOG NUMBER: 83-713
![Western blot analysis of various lysates using [KO Validated] PCIF1 Rabbit mAb (A24070) at 1:1000 dilution.| Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.| Lysates / proteins: 25 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit (RM00020).| Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC64786-01_WB_01_82-861.jpg)
CATALOG NUMBER: 82-861
![Western blot analysis of various lysates using [KO Validated] PML Rabbit mAb (A27714) at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC74393-01_WB_01_83-556.jpg)
CATALOG NUMBER: 83-556
![Western blot analysis of lysates from MCF7 cells, using [KO Validated] PRMT4/CARM1 Rabbit mAb (A21106) at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC52397-01_WB_01_81-876.jpg)
CATALOG NUMBER: 81-876
![Western blot analysis of various lysates using [KO Validated] PTEN Rabbit mAb (A19104) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC2643_WB_01_81-373.jpg)
CATALOG NUMBER: 81-373
![Western blot analysis of lysates from wild type (WT) and Rbm7 knockout (KO) mouse testis using [KO Validated] Rbm7 Rabbit mAb (A27655) at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.|WB samples for antibody validation are kindly provided by Dr. Shaorong Gao.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC73985-01_WB_02_83-542.jpg)
CATALOG NUMBER: 83-542
![Western blot analysis of lysates from wild type (WT) and Smad3 knockout (KO) 293T cells, using [KO Validated] Smad3 Rabbit mAb (A23491) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0490_KO-WB_01_82-639.jpg)
CATALOG NUMBER: 82-639
CATALOG NUMBER: 83-641
![Western blot analysis of lysates from wild type (WT) and Src knockout (KO) HeLa cells, using [KO Validated] Src Rabbit mAb (A19119) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0378_KO-WB_01_81-385.jpg)
CATALOG NUMBER: 81-385
![Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit mAb (A19567) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0046_KO-WB_01_81-495.jpg)
CATALOG NUMBER: 81-495
