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MAPK-Erk Signaling Pathway

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of various lysates using Phospho-Raf1-S259 Rabbit mAb at 1:1000 dilution. Both 293T cells and C6 cells were treated with Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Raf1-S259 Rabbit Recombinant Antibody (84-585)

CATALOG NUMBER: 84-585

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-Raf1-S621 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-Raf1-S621 Rabbit Recombinant Antibody (84-584)

CATALOG NUMBER: 84-584

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-RPS6-S235 Rabbit mAb at 1:3000 dilution. NIH/3T3 cells were treated with 20% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-RPS6-S235 Rabbit Recombinant Antibody (84-730)

CATALOG NUMBER: 84-730

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-S6 Ribosomal Protein -S235/236 Rabbit mAb at 1:6000 dilution. NIH/3T3 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.

Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Recombinant Antibody (84-645)

CATALOG NUMBER: 84-645

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S235/236 Rabbit mAb at 1:3000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with 10% FBS at 37℃ for 30 minutes. NIH/3T3 cells were treated with 10% FBS at 37℃ for 1.5 hours. PC-12 cells were treated with Anisomycin (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Recombinant Antibody (84-646)

CATALOG NUMBER: 84-646

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S240/244 Rabbit mAb at 1:20000 dilution. HeLa and NIH/3T3 cell were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. PC-12 cells were treated with 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Recombinant Antibody (84-647)

CATALOG NUMBER: 84-647

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S240/244 Rabbit mAb at 1:20000 dilution incubated overnight at 4℃. HeLa, NIH/3T3 and PC-12 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30 s.

Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Recombinant Antibody (84-761)

CATALOG NUMBER: 84-761

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells using Phospho-SRC Family-Y416 Rabbit mAb at 1:1000 dilution. C6 cells were treated with Pervanadate (0.5 mM) at 37℃ for 40 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-SRC Family-Y416 Rabbit Recombinant Antibody (84-668)

CATALOG NUMBER: 84-668

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-Src-Y419 Rabbit Recombinant Antibody (84-594)

CATALOG NUMBER: 84-594

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit mAb at 1:10000 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Src-Y529 Rabbit Recombinant Antibody (84-675)

CATALOG NUMBER: 84-675

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL