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MAPK-Erk Signaling Pathway

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of various lysates using Integrin β5/ITGB5 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.

Integrin β5/ITGB5 Rabbit Recombinant Antibody (82-599)

CATALOG NUMBER: 82-599

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and A549 cells (right) were surface-stained with Integrin β5/ITGB5 Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Integrin β5/ITGB5 Rabbit Recombinant Antibody (83-004)

CATALOG NUMBER: 83-004

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
FC
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Confocal imaging of A-431 cells using Integrin-β1/CD29 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Integrin-β1/CD29 Rabbit Recombinant Antibody (81-965)

CATALOG NUMBER: 81-965

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HepG2 cells, using IRS1 Rabbit mAb at 1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.

IRS1 Rabbit Recombinant Antibody (81-350)

CATALOG NUMBER: 81-350

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using IRS2 Rabbit mAbat 1:3000 dilution incubated overnight at 4°C.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. |Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|Negative control (NC): U-2 OS|Exposure time: 5 s.

IRS2 Rabbit Recombinant Antibody (83-139)

CATALOG NUMBER: 83-139

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using KRAS+HRAS+NRAS Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

KRAS+HRAS+NRAS Rabbit Recombinant Antibody (81-634)

CATALOG NUMBER: 81-634

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from Rat lung, using KRAS+HRAS+NRAS Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

KRAS+HRAS+NRAS Rabbit Recombinant Antibody (83-992)

CATALOG NUMBER: 83-992

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using MEK1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1min.

MEK1 Rabbit Recombinant Antibody (81-494)

CATALOG NUMBER: 81-494

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL