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Monoclonal

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Product Type
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Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with AKT2 using Phospho-AKT2-S474 Rabbit mAb at 1:1000 dilution. AKT2 transfected 293F cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-AKT2-S474(Akt2 Specific) Rabbit Recombinant Antibody (84-717)

CATALOG NUMBER: 84-717

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKα1-S496 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated with oligomycin (0. 5 μM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-AMPKα1-S496 Rabbit Recombinant Antibody (84-578)

CATALOG NUMBER: 84-578

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ASK1 using Phospho-ASK1-T845 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.293T cells transfected with ASK1 were treated by CIP (20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 5s.

Phospho-ASK1-T845 Rabbit Recombinant Antibody (84-771)

CATALOG NUMBER: 84-771

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from 293T cells using Phospho-ATM-S1981 Rabbit mAb at 1:8000 dilution incubated overnight at 4℃. 293T cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.

Phospho-ATM-S1981 Rabbit Recombinant Antibody (84-596)

CATALOG NUMBER: 84-596

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from 293T cells using Phospho-ATR-S428 Rabbit mAb at1:2000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90 s.

Phospho-ATR-S428 Rabbit Recombinant Antibody (84-661)

CATALOG NUMBER: 84-661

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-ATR-S428 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with UV at room temperature for 15-30 minutes and λ-pp (1 mM) for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.

Phospho-ATR-S428 Rabbit Recombinant Antibody (84-777)

CATALOG NUMBER: 84-777

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and A-549 cells transfected with Gas6 using Phospho-Axl-Y702 Rabbit mAb at 1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

Phospho-Axl-Y702 Rabbit Recombinant Antibody (84-705)

CATALOG NUMBER: 84-705

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb at 1:1000 dilution. C6 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-B-Raf-T401 Rabbit Recombinant Antibody (84-601)

CATALOG NUMBER: 84-601

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

Phospho-Bad-S112 Rabbit Recombinant Antibody (84-583)

CATALOG NUMBER: 84-583

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL