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Protein phosphorylation

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of recombinant Human p-Tau217 Protein using Phospho-Tau-T217 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 100ng μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 5s.

Phospho-Tau-T217 Rabbit Recombinant Antibody (84-672)

CATALOG NUMBER: 84-672

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-Tau-T231 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Phospho-Tau-T231 Rabbit Recombinant Antibody (84-548)

CATALOG NUMBER: 84-548

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from 293T cells using Phospho-ULK1-S555 Rabbit mAb at 1:8000 dilution incubated overnight at 4℃. 293T cells were treated by λ-PP mixed solution (62.5U) at 30℃ for 1 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.

Phospho-ULK1-S555 Rabbit Recombinant Antibody (84-741)

CATALOG NUMBER: 84-741

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-VASP-S157 Rabbit mAb at 1:4000 dilution. C6 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.

Phospho-VASP-S157 Rabbit Recombinant Antibody (84-749)

CATALOG NUMBER: 84-749

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit mAb at 1:3000 dilution. HEL cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-VASP-S157 Rabbit Recombinant Antibody (84-750)

CATALOG NUMBER: 84-750

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HEL cells using Phospho-VASP-S239 Rabbit mAb at 1:3000 dilution. HEL cells were treated with Forskolin (20 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-VASP-S239 Rabbit Recombinant Antibody (84-735)

CATALOG NUMBER: 84-735

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from A-431 lysates cells, using Phospho-VEGF Receptor 2-Y1175 Rabbit mAb at 1:3000 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-VEGF Receptor 2-Y1175 Rabbit Recombinant Antibody (84-682)

CATALOG NUMBER: 84-682

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from A-431 cells using Phospho-VEGF Receptor 2-Y1175 Rabbit mAb at 1:3900 dilution incubated overnight at 4℃. A-431 cells were treated by EGF (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 1s.

Phospho-VEGF Receptor 2-Y1175 Rabbit Recombinant Antibody (84-740)

CATALOG NUMBER: 84-740

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL