![Western blot analysis of various lysates using [KD Validated] DCK Rabbit mAb (A27081) at 1:2000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC65396-01_WB_01_83-449.jpg)
CATALOG NUMBER: 83-449
![Western blot analysis of various lysates using [KD Validated] DNMT1 Rabbit mAb (A19679) at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. |Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020)|Exposure time: 30 s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC51348-01_WB_02_81-575.jpg)
CATALOG NUMBER: 81-575
![Western blot analysis of lysates from 293F cells using [KD Validated] DYNC1H1 Rabbit mAb (A27292) at 1:13000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC72956-01_WB_01_83-506.jpg)
CATALOG NUMBER: 83-506
![Western blot analysis of lysates from Mouse heart using [KD Validated] ECH1 Rabbit mAb (A27290) at 1:12000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC71050-01_WB_01_83-504.jpg)
CATALOG NUMBER: 83-504
![Western blot analysis of lysates from wild type (WT) and ECHS1 knockdown (KD) HeLa cells using [KD Validated] ECHS1 Rabbit mAb (A27295) at 1:2000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 0.8s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC68644-01_WB_01_83-509.jpg)
CATALOG NUMBER: 83-509
![Western blot analysis of lysates from wild type (WT) and EFTUD2 knockdown (KD) HeLa cells using [KD Validated] EFTUD2 Rabbit mAb (A26306) at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC66934-01_WB_01_83-266.jpg)
CATALOG NUMBER: 83-266
![Western blot analysis of lysates from wild type(WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit mAb (A23452) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC56230-01_KO-WB_01_82-615.jpg)
CATALOG NUMBER: 82-615
![Western blot analysis of lysates from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using [KD Validated] EIF2AK1 Rabbit mAb (A24614) at 1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC62929-01_KO-WB_01_82-957.jpg)
CATALOG NUMBER: 82-957
![Western blot analysis of lysates from wild type (WT) and eIF4E knockdown (KD) 293T cells using [KD Validated] eIF4E Rabbit mAb (A25608) at 1:3000 dilution. |Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC66295-01_WB_01_83-122.jpg)
CATALOG NUMBER: 83-122
![Western blot analysis of various lysates using [KD Validated] eIF4EBP1 Rabbit mAb (A24691) at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC60753-03_WB_01_82-979.jpg)
CATALOG NUMBER: 82-979
