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Monoclonal

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
Conjugate
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Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-Tau-T231 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Phospho-Tau-T231 Rabbit Recombinant Antibody (84-548)

CATALOG NUMBER: 84-548

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-Trim24-S1043 Rabbit mAb at1:1000 dilution. Hela cells were treated with TGF-β (10 ng/ml) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

Phospho-Trim24-S1043 Rabbit Recombinant Antibody (84-634)

CATALOG NUMBER: 84-634

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from 293T cells using Phospho-ULK1-S555 Rabbit mAb at 1:8000 dilution incubated overnight at 4℃. 293T cells were treated by λ-PP mixed solution (62.5U) at 30℃ for 1 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.

Phospho-ULK1-S555 Rabbit Recombinant Antibody (84-741)

CATALOG NUMBER: 84-741

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using Phospho-VANGL2-S79/S82/S84 Rabbit mAb at1:1000 dilution. HeLa and C6 cells were treated with wnt(100ng/ml) for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-VANGL2-S79/S82/S84 Rabbit Recombinant Antibody (84-631)

CATALOG NUMBER: 84-631

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-VANGL2-T78/S79/S82 Rabbit mAb at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated with wnt(100ng/ml) for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

Phospho-VANGL2-T78/S79/S82 Rabbit Recombinant Antibody (84-630)

CATALOG NUMBER: 84-630

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-VASP-S157 Rabbit mAb at 1:4000 dilution. C6 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.

Phospho-VASP-S157 Rabbit Recombinant Antibody (84-749)

CATALOG NUMBER: 84-749

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL