![Western blot analysis of lysates from wild type (WT) and AK2 knockout (KO) HeLa cells using [KO Validated] AK2 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 5 s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC2916_WB_01_81-805.jpg)
CATALOG NUMBER: 81-805
![Western blot analysis of various lysates using [KO Validated] AKT1 Rabbit mAb at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC51585-01_WB_01_82-401.jpg)
CATALOG NUMBER: 82-401
![Western blot analysis of various lysates using [KO Validated] AMPKα1 Rabbit mAb at 1:12000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC73667-01_WB_01_83-576.jpg)
CATALOG NUMBER: 83-576
![Western blot analysis of lysates from wild type (WT) and AMPKα1 knockout (KO) HeLa cells using [KO Validated] AMPKα1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC73664-01_WB_01_83-629.jpg)
CATALOG NUMBER: 83-629
![Western blot analysis of lysates from wild type (WT) and ATG16L1 knockout (KO) HeLa cells using [KO Validated] ATG16L1 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0812_WB_02_83-727.jpg)
CATALOG NUMBER: 83-727
![Western blot analysis of lysates from HT-1080 cells using [KO Validated] Bax Rabbit mAb at 1:10000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC5006-06-02_WB_01_81-298.jpg)
CATALOG NUMBER: 81-298
![Western blot analysis of lysates from wild type (WT) and Beclin1 knockout (KO) 293T(KO) cells, using [KO Validated] Beclin 1 Rabbit mAb at 1:30000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC52867-01_KO-WB_01_82-214.jpg)
CATALOG NUMBER: 82-214
![Western blot analysis of various lysates, using [KO Validated] Beclin 1 Rabbit mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC52858-01_WB_01_82-216.jpg)
CATALOG NUMBER: 82-216
![Western blot analysis of lysates from wild type (WT) and Calnexin knockout (KO) HeLa cells using [KO Validated] Calnexin Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC75323-01_WB_01_83-584.jpg)
CATALOG NUMBER: 83-584
![Western blot analysis of various lysates using [KO Validated] Calreticulin Mouse mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/AMC50035-01_WB_01_83-467.jpg)
CATALOG NUMBER: 83-467
