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![Immunoprecipitation of Rab12 from 250 µg of extracts of A549 wild-type (WT) or A549 Rab12 knock-out (Rab12 KO) cells was performed using 1.5 µg of Rab12 Rabbit mAb [MJFF-A-3F6] conjugated to MyOne™ Epoxy Dynabeads®. Negative Control IP (Control IgG) was performed using MyOne™ Epoxy Dynabeads® conjugated with Bovine Serum Albumin. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 4% of the total input. Western blot analysis of immunoprecipitates was conducted using Rab12 Rabbit mAb [MJFF-A-3F6] at a dilution of 1:1000.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC5165-01_IP_01_83-502.jpg)
