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WB

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
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[KO Validated] Glutamine Synthetase (GLUL) Rabbit Recombinant Antibody (81-550)

CATALOG NUMBER: 81-550

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and GRK2 knockout (KO) HeLa cells, using [KO Validated] GRK2 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30 s.

[KO Validated] GRK2 Rabbit Recombinant Antibody (83-924)

CATALOG NUMBER: 83-924

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and GSK3α knockout (KO) HeLa cells, using [KO Validated] GSK3α Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

[KO Validated] GSK3α Rabbit Recombinant Antibody (81-341)

CATALOG NUMBER: 81-341

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from Hep G2 cells, using HADHA Rabbit mAb at 1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

[KO Validated] HADHA Rabbit Recombinant Antibody (82-953)

CATALOG NUMBER: 82-953

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from K-562, using 3 μg of [KO Validated] HDAC1 Rabbit mAb and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

[KO Validated] HDAC1 Rabbit Recombinant Antibody (83-308)

CATALOG NUMBER: 83-308

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, ELISA, IF-P, IF/ICC, IHC-P, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using [KO Validated] HIF1AN/FIH-1 Rabbit mAb at 1:16000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.

[KO Validated] HIF1AN/FIH-1 Rabbit Recombinant Antibody (83-513)

CATALOG NUMBER: 83-513

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using [KO Validated] HIF1AN/FIH1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.

[KO Validated] HIF1AN/FIH1 Rabbit Recombinant Antibody (83-749)

CATALOG NUMBER: 83-749

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Histone H2A.Z Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

[KO Validated] Histone H2A.Z Rabbit Recombinant Antibody (83-959)

CATALOG NUMBER: 83-959

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

[KO Validated] HK1 Rabbit Recombinant Antibody (81-037)

CATALOG NUMBER: 81-037

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IF/ICC, IHC-P, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using HPRT11 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

[KO Validated] HPRT1 Rabbit Recombinant Antibody (84-237)

CATALOG NUMBER: 84-237

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL