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Affinity purification

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Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
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Western blot analysis of various lysates using MonoMethyl-Histone H3-K9 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

MonoMethyl-Histone H3-K9 Rabbit Recombinant Antibody (81-708)

CATALOG NUMBER: 81-708

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, CUT&Tag, DB, ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using MonoMethyl-Histone H3-K9 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

MonoMethyl-Histone H3-K9 Rabbit Recombinant Antibody (82-072)

CATALOG NUMBER: 82-072

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Western blot analysis of various lysates using MonoMethyl-Histone H3-R2 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): H3 protein.|Exposure time: 60 s.

MonoMethyl-Histone H3-R2 Rabbit Recombinant Antibody (81-554)

CATALOG NUMBER: 81-554

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
CUT&Tag, DB, ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Western blot analysis of various lysates, using MonoMethyl-Histone H4-K20 Rabbit mAb at 1:900 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

MonoMethyl-Histone H4-K20 Rabbit Recombinant Antibody (82-299)

CATALOG NUMBER: 82-299

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Mouse anti DDDDK-Tag using Mouse anti DDDDK-Tag mAb at 1:20000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 45s.

Mouse anti DDDDK-Tag Recombinant Antibody (84-509)

CATALOG NUMBER: 84-509

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, FC (intra), IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of insect expressed GFP protein using Mouse anti GFP-Tag mAb at different dilution.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

Mouse anti GFP-Tag Recombinant Antibody (84-512)

CATALOG NUMBER: 84-512

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and Insect cells transfected with GFP-Tag using Mouse anti GFP-Tag mAb at 1:25000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10ng μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 20s.

Mouse anti GFP-Tag Recombinant Antibody (84-542)

CATALOG NUMBER: 84-542

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and insert cells transfected with GFP-Tag using GFP-Tag Mouse mAb at 1:18000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10 ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.

Mouse anti GFP-Tag Recombinant Antibody (84-543)

CATALOG NUMBER: 84-543

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of over-expressed HA-tagged protein in 293T cell using Mouse anti HA-Tag mAb at different dilution. Each lane was loaded with 2 ug cell lysate.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

Mouse anti HA-Tag Recombinant Antibody (84-510)

CATALOG NUMBER: 84-510

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with His-Tag using Mouse anti His-Tag mAb at 1:30000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 5 s.

Mouse anti His-Tag Recombinant Antibody (84-508)

CATALOG NUMBER: 84-508

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL