Apoptosis

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
Conjugate
Product Badges
Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb at 1:10000 dilution incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated with λPP (2 U/μL) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-STAT3-Y705 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-STAT3-Y705 Rabbit mAb at 1:20000 dilution. HeLa cells were treated with IFN-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from TF-1 cells, using Phospho-STAT5A-Y694 Rabbit mAb at 1:1000 dilution. TF-1 cells were treated with GM-CSF (25 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-Stathmin 1-S25 Rabbit mAb at 1:1000 dilution. C6 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis lysates from SH-SY5Y, using Phospho-Tau (S214) Rabbit mAb at 1:1000 dilution. SH-SY5Y cells were treated with Okadaic Acid (100 nM) at 37℃ for 1 hour and Calyculin A (100nM) at 37℃ for 30 minutes after serum-starvation overnight.℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from Mouse brain, using Phospho-Tau-S396 Rabbit mAb at 1:1000 dilution. Mouse brain were treated by CIP(20μL/400μL) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg  per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from rat brain, using Phospho-Tau-S404 Rabbit mAb at  1:5000 dilution. Rat brain cells were treated with λ-PP mixed solution (1ul) at 30℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from 293T cells transfected with CDK5+CDK5R1+Tau and  CDK5+CDK5R1+mutant Tau(T181/T217A) using Phospho-Tau-T181 Rabbit mAb at1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL