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![Western blot analysis of lysates from HeLa cells, using [KD Validated] glucose-6-phosphateisomerase Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0997_WB_01_83-909.jpg)
![Western blot analysis of lysates from wild type (WT) and GPI knockdown (KD) HeLa cells using [KD Validated] GPI Rabbit mAb at 1:6000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 5 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC74854-01_WB_03_83-550.jpg)
![Western blot analysis of various lysates using [KD Validated] LDHA Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC70631-01_WB_01_83-309.jpg)
![Western blot analysis of lysates from wild type (WT) and MCT1 knockdown (KD) 293T cells using [KD Validated] MCT1 Rabbit mAb at 1:2000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC72781-01_WB_01_83-499.jpg)
![Western blot analysis of various lysates, using [KD Validated] PKA C-alpha (PRKACA) Mouse mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/AMC0275R-01_WB_01_81-297.jpg)
![Western blot analysis of lysates from wild type (WT) and PKA C-alpha (PRKACA) knockdown (KD) 293T cells, using [KD Validated] PKA C-alpha (PRKACA) Mouse mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/AMC0275-02-1_KO-WB_01_81-993.jpg)
![Western blot analysis of lysates from wild type (WT) and TKT knockdown (KD) HeLa cells using [KD Validated] TKT/Transketolase Rabbit mAb at 1:13000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC69667-01_WB_01_83-472.jpg)
![Western blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0256_KO-WB_01_81-037.jpg)
![Western blot analysis of lysates from wild type (WT) and Lactate Dehydrogenase B/LDH-B knockout (KO) 293T cells using [KO Validated] Lactate Dehydrogenase B/LDH-B Rabbit mAb at 1:12000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC71823-01_WB_01_83-531.jpg)
![Western blot analysis of lysates from MCF7 cells, using [KO Validated] LDHA Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC2669_WB_01_81-082.jpg)
