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Cardiovascular

Categories

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from Jurkat cells, using Phospho-Akt-T308 Rabbit mAb at1:2000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-Akt-T308 Rabbit Recombinant Antibody (84-648)

CATALOG NUMBER: 84-648

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb at 1:1000 dilution. MCF7 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-AKT1-S124 Rabbit Recombinant Antibody (84-561)

CATALOG NUMBER: 84-561

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb at 1:1000 dilution. MCF7 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-AKT1-S129 Rabbit Recombinant Antibody (84-560)

CATALOG NUMBER: 84-560

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb at 1:1000 dilution. C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with PDGF (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

Phospho-AKT1-T450 Rabbit Recombinant Antibody (84-559)

CATALOG NUMBER: 84-559

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with AKT2 using Phospho-AKT2-S474 Rabbit mAb at 1:1000 dilution. AKT2 transfected 293F cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-AKT2-S474(Akt2 Specific) Rabbit Recombinant Antibody (84-717)

CATALOG NUMBER: 84-717

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKα1-S496 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated with oligomycin (0. 5 μM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-AMPKα1-S496 Rabbit Recombinant Antibody (84-578)

CATALOG NUMBER: 84-578

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from Mouse brain using Phospho-Camk2-T286 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. Mouse brain treated with CIP (1U/ul) at 37℃ for 1 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.

Phospho-Camk2-T286 Rabbit Recombinant Antibody (84-683)

CATALOG NUMBER: 84-683

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human heart using Phospho-Cardiac Troponin I (TNNI3)-S23/S24 Rabbit mAb at dilution of 1:700 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Phospho-Cardiac Troponin I (TNNI3)-S23/S24 Rabbit Recombinant Antibody (84-688)

CATALOG NUMBER: 84-688

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from MCF7 cells using Phospho-CEBPB-T235 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. MCF7 cells were treated with U0126 (20 μM) for 48 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90s.

Phospho-CEBPB-T235 Rabbit Recombinant Antibody (84-772)

CATALOG NUMBER: 84-772

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-EGFR-Y1068 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 60s.

Phospho-EGFR-Y1068 Rabbit Recombinant Antibody (84-547)

CATALOG NUMBER: 84-547

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL