Cell Adhesion

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and A549 cells (right) were surface-stained with Integrin β5/ITGB5 Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
FC
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Confocal imaging of A-431 cells using Integrin-β1/CD29 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using IQGAP1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using IQGAP2 Rabbit mAb at 1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates, using JAM-A/CD321/F11R Rabbit mAb at  1:8000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from A-431 cells using JAM-A/CD321/F11R Rabbit mAb at 1:2000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using K-Cadherin (CDH6) Rabbit mAb at 1:3000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using LAMC1 Rabbit mAb at 1:3000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): K-562.|Exposure time: 5s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using LAMC1 Rabbit mAb at 1:3000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using LEF1 Rabbit mAb at 1:7000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): U-937|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL