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Cell Cycle Control-G1 S Checkpoint

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Categories

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S9 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

Phospho-p53-S9 Rabbit Recombinant Antibody (84-563)

CATALOG NUMBER: 84-563

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from 293T cells, using Phospho-p53-T55 Rabbit mAb at 1:1000 dilution. 293T cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-p53-T55 Rabbit Recombinant Antibody (84-564)

CATALOG NUMBER: 84-564

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-RB-S780 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-550)

CATALOG NUMBER: 84-550

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RB-S780 Rabbit mAb at 1:10000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 90s.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-663)

CATALOG NUMBER: 84-663

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-RB-S780 Rabbit mAb at 1:19000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-698)

CATALOG NUMBER: 84-698

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-RB-S795 Rabbit mAb at 1:1000 dilution. HT-29 cells were treated with Nocodazole (100 ng/ml) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

Phospho-RB-S795 Rabbit Recombinant Antibody (84-681)

CATALOG NUMBER: 84-681

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from Jurkat cells using RB Rabbit mAb at 1:80000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.

RB Rabbit Recombinant Antibody (83-625)

CATALOG NUMBER: 83-625

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using RB Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

RB Rabbit Recombinant Antibody (83-627)

CATALOG NUMBER: 83-627

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Confocal imaging of U-2 OS cells using RB Rabbit mAb(Red). The cells were counterstained with α-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

RB Rabbit Recombinant Antibody (83-724)

CATALOG NUMBER: 83-724

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL