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![Western blot analysis of lysates from wild type (WT) and HSP27/HSPB1 knockdown (KD) HeLa cells using [KD Validated] HSP27/HSPB1 Rabbit mAb at 1:13000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC75566-01_WB_01_83-586.jpg)
![Western blot analysis of various lysates using [KD Validated] Vinculin Rabbit mAb at 1:21000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC75475-01_WB_01_83-600.jpg)
