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![Western blot analysis of lysates from HeLa cells using [KO Validated] TRIM21/SS-A Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC64169-01_WB_01_82-991.jpg)
![Western blot analysis of lysates from Jurkat cells using [KO Validated] TRIM21/SS-A Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC74407-02_WB_01_83-533.jpg)
![Western blot analysis of lysates from wild type(WT) and TRIM25 knockout (KO) 293T(KO) cells, using [KO Validated] TRIM25 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0971_KO-WB_01_83-888.jpg)
![Western blot analysis of various lysates, using [KO Validated] Von Hippel Lindau/VHL Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC59984-01_WB_01_82-533.jpg)
![Western blot analysis of lysates from wild type (WT) and α-Catenin knockout (KO) 293T cells, using [KO Validated] α-Catenin Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0348_KO-WB_01_81-302.jpg)
