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![Western blot analysis of lysates from PC-12 cells, using [KD Validated] USF1 Rabbit mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC58148-01_WB_01_82-627.jpg)
![Western blot analysis of various lysates using [KO Validated] AMPKα1 Rabbit mAb at 1:12000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73667-01_WB_01_83-576.jpg)
![Western blot analysis of lysates from wild type (WT) and AMPKα1 knockout (KO) HeLa cells using [KO Validated] AMPKα1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73664-01_WB_01_83-629.jpg)
![Western blot analysis of various lysates using [KO Validated] HIF1AN/FIH-1 Rabbit mAb at 1:16000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73051-01_WB_01_83-513.jpg)
![Western blot analysis of lysates from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using [KO Validated] HIF1AN/FIH1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0826_KO-WB_01_83-749.jpg)
![Western blot analysis of lysates from wild type (WT) and PHD2/EGLN1 knockout (KO) 293T cells, using [KO Validated] PHD2/EGLN1 Rabbit mAb at 1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 8s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC52542-01_KO-WB_01_81-972.jpg)
