Immunology Innate Immunity Macrophage / Inflamm.Immunology Innate Immunity Chemokines Beta Chemokines (CC)Kits/ Lysates/ Other Kits ELISA Kits ELISA Kits Cytokines and cytokine receptors ELISA kitsIm

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from THP-1 cells using CCL4/MIP-1 beta Rabbit mAb at 1:11000 dilution incubated at room temperature for 1.5 hours. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight, LPS (1 μg/mL) and BFA (300 ng/mL) for 8 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL