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Invasion and Metastasis

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Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from Jurkat cells using Cleaved Caspase-7 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Jurkat cells were treated with Etoposide (25 μM) at 37℃ for 5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 30s.

Cleaved Caspase-7 Rabbit Recombinant Antibody (82-481)

CATALOG NUMBER: 82-481

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from GSDMD knockout (KO) HeLa cells and GSDMD knockout (KO) HeLa cells transfected with LPS using Cleaved IL-18 (Tyr37) Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time:60s.|WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Cleaved IL-18 (Tyr37) Rabbit Recombinant Antibody (82-854)

CATALOG NUMBER: 82-854

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Cofilin Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Cofilin Rabbit Recombinant Antibody (83-315)

CATALOG NUMBER: 83-315

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Collagen IV Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): U-937.|Exposure time: 45s.

Collagen IV Rabbit Recombinant Antibody (83-052)

CATALOG NUMBER: 83-052

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Confocal imaging of U266 cells using Collagen IV Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Collagen IV Rabbit Recombinant Antibody (83-440)

CATALOG NUMBER: 83-440

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Contactin 2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Contactin 2 Rabbit Recombinant Antibody (84-107)

CATALOG NUMBER: 84-107

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates, using COUP-TFII/NR2F2 Rabbit mAb at 1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC):MOLT-4|Exposure time: 45s.

COUP-TFII/NR2F2 Rabbit Recombinant Antibody (82-970)

CATALOG NUMBER: 82-970

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using CXCL10/IP-10 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with IFN-γ (100 ng/mL), LPS (1 μg/mL) and Brefeldin A (300 ng/mL) for 8 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 10s.

CXCL10/IP-10 Rabbit Recombinant Antibody (82-041)

CATALOG NUMBER: 82-041

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using CXCL10/IP-10 Rabbit mAb at 1:10000 dilution incubated overnight at 4℃. A549 cells and HT-29 cells were treated with TNF-α (20ng/mL) for 6 hours, Brefeldin A (300ng/mL) for 3 hours and IFN-γ (50ng/mL) for 8 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 5s.

CXCL10/IP-10 Rabbit Recombinant Antibody (83-517)

CATALOG NUMBER: 83-517

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL