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mTOR Signaling Pathway

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Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from C2C12 cells, using Phospho-IRS1-S612/S636 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated with Insulin (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-IRS1-S612/S636 Rabbit Recombinant Antibody (84-747)

CATALOG NUMBER: 84-747

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using Phospho-mTOR-S2481 at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated with IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-mTOR-S2481 Rabbit Recombinant Antibody (84-557)

CATALOG NUMBER: 84-557

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb at 1:1000 dilution. NIH/3T3 and C6 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60S.

Phospho-p70 S6 Kinase 1-T421/S424 Rabbit Recombinant Antibody (84-552)

CATALOG NUMBER: 84-552

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.

Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit Recombinant Antibody (84-621)

CATALOG NUMBER: 84-621

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 Rabbit mAb at 1:2000 dilution. PC-3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 120s.

Phospho-PDK1/PDPK1-S241 Rabbit Recombinant Antibody (84-660)

CATALOG NUMBER: 84-660

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from C2C12 cells, using Phospho-PKC alpha-T638 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-PKC alpha-T638 Rabbit Recombinant Antibody (84-603)

CATALOG NUMBER: 84-603

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from 293T cells, using Phospho-PKC alpha-T638 Rabbit mAb at 1:7000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

Phospho-PKC alpha-T638 Rabbit Recombinant Antibody (84-674)

CATALOG NUMBER: 84-674

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-PTEN-S380 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by λPP mixed solution (1uL) at 30℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 180s.

Phospho-PTEN-S380 Rabbit Recombinant Antibody (84-574)

CATALOG NUMBER: 84-574

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-PTEN-S380/T382/T383 Rabbit mAb at 1:2000 dilution. Hela,NIH/3T3,and PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

Phospho-PTEN-S380/T382/T383 Rabbit Recombinant Antibody (84-655)

CATALOG NUMBER: 84-655

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL