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![Western blot analysis of lysates from HT-1080 cells using [KO Validated] Bax Rabbit mAb at 1:10000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC5006-06-02_WB_01_81-298.jpg)
![Western blot analysis of lysates from wild type (WT) and Calnexin knockout (KO) HeLa cells using [KO Validated] Calnexin Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC75323-01_WB_01_83-584.jpg)
![Western blot analysis of various lysates using [KO Validated] Caspase-8 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0028_WB_01_81-483.jpg)
![Western blot analysis of various lysates, using [KO Validated] CDK5 Rabbit mAb at1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC53344-01_WB_01_82-647.jpg)
![Western blot analysis of lysates from wild type (WT) and Gasdermin E (N terminal) knockout (KO) HeLa cells using [KO Validated] Cleaved Gasdermin E (N terminal) Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and GSDME knockout (KO) HeLa cells were treated with TNF-α (50ng/mL) and CHX (10μg/mL) at 37℃ for 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.|WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS](https://www.prosciantibodies.com/wp-content/uploads/products/ARC70388-01_WB_05_83-232.jpg)
![Western blot analysis of lysates from Rat brain using [KO Validated] CTIP1/BCL11A Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC1486_WB_02_84-339.jpg)
![Western blot analysis of various lysates using [KO Validated] FGFR2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0480_WB_01_81-334.jpg)