NF-kB Signaling Pathway

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from Recombinant Human IL-1 alpha Protein  using IL-1 alpha Rabbit mAb at 1:2000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10ng,5ng μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of Recombinant Mouse IL-1 beta Protein , using IL1β Rabbit mAb at1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10ng/5ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using IL1β Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with LPS (0.5 μg/mL) for 2 hours after serum starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 5 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from U-87 MG cells, using IL1β Rabbit mAb at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using IL1β Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours and Brefeldin A for 3 hours of stimulation. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Insulin Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL