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Protein phosphorylation

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Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from 293T cells, using Phospho-PKC alpha-T638 Rabbit mAb at 1:7000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

Phospho-PKC alpha-T638 Rabbit Recombinant Antibody (84-674)

CATALOG NUMBER: 84-674

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-PKR/EIF2AK2-T446 Rabbit Recombinant Antibody (84-613)

CATALOG NUMBER: 84-613

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit mAb at 1:3000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by PDGF-BB (100 ng/ml) for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 30s.

Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit Recombinant Antibody (84-742)

CATALOG NUMBER: 84-742

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit mAb at 1:2000 dilution. NIH/3T3 cells were treated with PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit Recombinant Antibody (84-652)

CATALOG NUMBER: 84-652

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from Raji cells using Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours. Raji cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 1s.

Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit Recombinant Antibody (84-774)

CATALOG NUMBER: 84-774

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from Raji cells using Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit mAb at 1:5000 dilution incubated overnight at 4°C. Raji cells were treated by Pervanadate (500 μM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit Recombinant Antibody (84-755)

CATALOG NUMBER: 84-755

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-PLK1-T210 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with Nocodazoleat (100 ng/mL) at 37 ℃ for 16 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90 s.

Phospho-PLK1-T210 Rabbit Recombinant Antibody (84-592)

CATALOG NUMBER: 84-592

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from MCF7 cells using Phospho-POLR2A CTD-S2 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. MCF7 cells were treated with λpp (2 U/ul) at 30℃ for 1 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 20 s.

Phospho-POLR2A CTD-S2 Rabbit Recombinant Antibody (84-572)

CATALOG NUMBER: 84-572

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, ChIP-seq, CUT&Tag, ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb at 1:1000 dilution. MCF7 cells were treated with Doxorubicin (0. 5 uM) at 37℃ for 24 hours or treated with nocodazole (50 ng/mL) at 37℃ for 20 hours or treated with CIP(20uL/400ul) at 37℃ for 1 hour. C2C12 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 1s.

Phospho-POLR2A CTD-S5 Rabbit Recombinant Antibody (84-573)

CATALOG NUMBER: 84-573

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-PPP1CA-T320 Rabbit mAb at 1:2000 dilution. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.

Phospho-PPP1CA-T320 Rabbit Recombinant Antibody (84-711)

CATALOG NUMBER: 84-711

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL