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RNA Binding

Categories

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3s.

Phospho-HSP27/HSPB1-S82 Rabbit Recombinant Antibody (84-597)

CATALOG NUMBER: 84-597

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human esophagus using Phospho-Nucleolin-T76 Rabbit mAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Phospho-Nucleolin-T76 Rabbit Recombinant Antibody (84-637)

CATALOG NUMBER: 84-637

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-RPS6-S235 Rabbit mAb at 1:3000 dilution. NIH/3T3 cells were treated with 20% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-RPS6-S235 Rabbit Recombinant Antibody (84-730)

CATALOG NUMBER: 84-730

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-S6 Ribosomal Protein -S235/236 Rabbit mAb at 1:6000 dilution. NIH/3T3 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.

Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Recombinant Antibody (84-645)

CATALOG NUMBER: 84-645

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S235/236 Rabbit mAb at 1:3000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with 10% FBS at 37℃ for 30 minutes. NIH/3T3 cells were treated with 10% FBS at 37℃ for 1.5 hours. PC-12 cells were treated with Anisomycin (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Recombinant Antibody (84-646)

CATALOG NUMBER: 84-646

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S240/244 Rabbit mAb at 1:20000 dilution. HeLa and NIH/3T3 cell were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. PC-12 cells were treated with 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Recombinant Antibody (84-647)

CATALOG NUMBER: 84-647

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S240/244 Rabbit mAb at 1:20000 dilution incubated overnight at 4℃. HeLa, NIH/3T3 and PC-12 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30 s.

Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Recombinant Antibody (84-761)

CATALOG NUMBER: 84-761

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-YWHAZ -Ser58+YWHAH -Ser59+YWHAG-Ser59+YWHAB -Ser60 Rabbit mAb at 1:13000 dilution incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated with IBMX (50μM) and Forskolin (10 μM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-YWHAZ -Ser58+YWHAH -Ser59+YWHAG-Ser59+YWHAB -Ser60 Rabbit Recombinant Antibody (84-767)

CATALOG NUMBER: 84-767

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using PKM1-specific Rabbit mAb at 1:9000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): NIH/3T3|Exposure time: 1s.

PKM1-specific Rabbit Recombinant Antibody (81-859)

CATALOG NUMBER: 81-859

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates, using PKM2-specific Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

PKM2-specific Rabbit Recombinant Antibody (82-243)

CATALOG NUMBER: 82-243

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL