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Tyrosine kinases

Categories

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from NIH/3T3 cells using Phospho-FAK-Y576/Y577 Rabbit mAb at 1:16000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 10s.

Phospho-FAK-Y576/Y577 Rabbit Recombinant Antibody (84-764)

CATALOG NUMBER: 84-764

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from A-431 cells, using Phospho-HER2/ErbB2-Y1139 at 1:500 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-HER2/ErbB2-Y1139 Rabbit Recombinant Antibody (84-635)

CATALOG NUMBER: 84-635

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HuH-7 cells using Phospho-JAK2-Y1007/1008 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. HuH-7 cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 5s.

Phospho-JAK2-Y1007/1008 Rabbit Recombinant Antibody (84-775)

CATALOG NUMBER: 84-775

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y397 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Lyn-Y397 Rabbit Recombinant Antibody (84-606)

CATALOG NUMBER: 84-606

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb at 1:1000 dilution. Both HeLa cells and C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 1min.

Phospho-MEK1-S298 Rabbit Recombinant Antibody (84-588)

CATALOG NUMBER: 84-588

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 60 s.

Phospho-MEK1-T292 Rabbit Recombinant Antibody (84-589)

CATALOG NUMBER: 84-589

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 Rabbit mAb at1:2000 dilution. HeLa cells were treated with HGF (40 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 90s.

Phospho-Met-Y1234/Y1235 Rabbit Recombinant Antibody (84-651)

CATALOG NUMBER: 84-651

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells using Phospho-SRC Family-Y416 Rabbit mAb at 1:1000 dilution. C6 cells were treated with Pervanadate (0.5 mM) at 37℃ for 40 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-SRC Family-Y416 Rabbit Recombinant Antibody (84-668)

CATALOG NUMBER: 84-668

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-Src-Y419 Rabbit Recombinant Antibody (84-594)

CATALOG NUMBER: 84-594

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL