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![Western blot analysis of lysates from HeLa cells using [KD Validated] ATF6 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. HeLa cells were treated with Tunicamycin (2 μg/ml) for 1 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC75670-01_WB_01_83-595.jpg)
