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IF/ICC

Categories

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Conjugate
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Mouse anti DDDDK-Tag using Mouse anti DDDDK-Tag mAb at 1:20000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 45s.

Mouse anti DDDDK-Tag Recombinant Antibody (84-509)

CATALOG NUMBER: 84-509

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, FC (intra), IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and insert cells transfected with GFP-Tag using GFP-Tag Mouse mAb at 1:18000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10 ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.

Mouse anti GFP-Tag Recombinant Antibody (84-543)

CATALOG NUMBER: 84-543

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of over-expressed HA-tagged protein in 293T cell using Mouse anti HA-Tag mAb at different dilution. Each lane was loaded with 2 ug cell lysate.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

Mouse anti HA-Tag Recombinant Antibody (84-510)

CATALOG NUMBER: 84-510

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with His-Tag using Mouse anti His-Tag mAb at 1:30000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 5 s.

Mouse anti His-Tag Recombinant Antibody (84-508)

CATALOG NUMBER: 84-508

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NLK-Myc (C-terminal) or PTEN-Myc (C-terminal) using Mouse anti Myc-Tag mAb at 1:10000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 30s.

Mouse anti Myc-Tag Recombinant Antibody (84-511)

CATALOG NUMBER: 84-511

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of various lysates, using MRPS15 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

MRPS15 Rabbit Recombinant Antibody (84-384)

CATALOG NUMBER: 84-384

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using MSH6 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

MSH6 Rabbit Recombinant Antibody (84-239)

CATALOG NUMBER: 84-239

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using mSin3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

mSin3A Rabbit Recombinant Antibody (82-811)

CATALOG NUMBER: 82-811

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using MSMB Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

MSMB Rabbit Recombinant Antibody (83-844)

CATALOG NUMBER: 83-844

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using MTAP Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

MTAP Rabbit Recombinant Antibody (81-778)

CATALOG NUMBER: 81-778

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL