Inhibition

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Conjugate
Flow cytometry: PD-L1/CD274 expressing 293F cells were stained with control IgG or PD-L1/CD274 Rabbit mAb.|Primary:PD-L1/CD274 Rabbit mAb at 1:100 dilution|Secondary:1:1000 goat anti-rabbit IgG FITC
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, Inhibition
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and PD-L1/CD274 knockout (KO) A549 cells using PD-L1/CD274 Rabbit mAb at 1:8000  dilution incubated overnight at 4℃. Wild type (WT) and PD-L1/CD274 knockout (KO) A549 cells were treated with IFNG (100 ng/mL) at 37℃ for 48 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, Inhibition, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL