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DNA Damage Repair

Categories

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from 293T cells, using Phospho-p53-T55 Rabbit mAb at 1:1000 dilution. 293T cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-p53-T55 Rabbit Recombinant Antibody (84-564)

CATALOG NUMBER: 84-564

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb at 1:1000 dilution. NIH/3T3 and C6 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60S.

Phospho-p70 S6 Kinase 1-T421/S424 Rabbit Recombinant Antibody (84-552)

CATALOG NUMBER: 84-552

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from MCF7 cells using Phospho-POLR2A CTD-S2 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. MCF7 cells were treated with λpp (2 U/ul) at 30℃ for 1 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 20 s.

Phospho-POLR2A CTD-S2 Rabbit Recombinant Antibody (84-572)

CATALOG NUMBER: 84-572

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, ChIP-seq, CUT&Tag, ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb at 1:1000 dilution. MCF7 cells were treated with Doxorubicin (0. 5 uM) at 37℃ for 24 hours or treated with nocodazole (50 ng/mL) at 37℃ for 20 hours or treated with CIP(20uL/400ul) at 37℃ for 1 hour. C2C12 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 1s.

Phospho-POLR2A CTD-S5 Rabbit Recombinant Antibody (84-573)

CATALOG NUMBER: 84-573

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-RB-S780 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-550)

CATALOG NUMBER: 84-550

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RB-S780 Rabbit mAb at 1:10000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 90s.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-663)

CATALOG NUMBER: 84-663

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-RB-S780 Rabbit mAb at 1:19000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-698)

CATALOG NUMBER: 84-698

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-RB-S795 Rabbit mAb at 1:1000 dilution. HT-29 cells were treated with Nocodazole (100 ng/ml) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

Phospho-RB-S795 Rabbit Recombinant Antibody (84-681)

CATALOG NUMBER: 84-681

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-RPA2-Ser33 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Camptothecin (1 μM) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-RPA2-Ser33 Rabbit Recombinant Antibody (84-736)

CATALOG NUMBER: 84-736

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-RPA2-T21 Rabbit mAb at 1:1000 dilution. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-RPA2-T21 Rabbit Recombinant Antibody (84-600)

CATALOG NUMBER: 84-600

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL