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![Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] Phospho-p53-S15 Rabbit mAb at 1:3000 dilution incubated overnight at 4℃. 293T cells and P53 knockout (KO) 293T cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC63388-01_WB_03_84-745.jpg)