FC (intra)

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from wild type(WT) and LAMP1 knockout (KO) HeLa(KO) cells, using [KO Validated] LAMP1/CD107a Rabbit mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra), IF/ICC, IHC-P, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from A549 cells using [KO Validated] LAMP2 Rabbit mAb at 1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time:10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra), IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Flow cytometry:1X10^6 HAP1 cells (Low Expression, Left) and Daudi cells (Right) were intracellularly-stained with BCL6 Rabbit mAb(2 μg/mL,orange line) or PSFlo 488 Rabbit IgG isotype control,followed by FITC conjugated goat anti-rabbit pAb (1:200 dilution) staining.Non-fluorescently stained cells were used as blank control (red line).
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra)
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Cytokeratin 19 (KRT19) Rabbit mAb at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra), IF-P, IF/ICC, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates, using ESRα Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): SK-BR-3|Exposure time: 3s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, ELISA, FC (intra), IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using GATA3 Rabbit mAb at 1:7000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): U-937|Exposure time: 1s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra), IF-P, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates, using HSP40 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra), WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of Active Recombinant Human IFN-gamma Protein  using IFN-gamma Rabbit mAb at 1:1000 dilution incubated overnight at 4°C.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 0.1ng - 10ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra), WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Flow cytometry: 1X10^6 CHO cells (negative control,left) and CHO (Transfection,right) cells were intracellularly-stained with IL-13 Rabbit mAb or PE Rabbit IgG isotype control, followed by PE Donkey anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra)
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from 293T cells transfected with IL12B using IL12B Rabbit mAb at 1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC (intra), IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL