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HRP

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Species Reactivity
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Dose response curve of HRP conjugated Rabbit Anti-Mouse IgE mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgE mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Anti-Mouse IgE mAb has highly specific recognition of Mouse IgE while no or minimal cross reactivity to Mouse IgG1、Mouse IgG2a、Mouse IgG2b、Mouse IgG3、Mouse IgM、Mouse IgA、Human IgG、Rat IgG.

HRP conjugated Rabbit Anti-Mouse IgE (Fc) Recombinant Antibody (84-793)

CATALOG NUMBER: 84-793

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
List Size:
100 uL
Dose response curve of HRP conjugated Mouse IgG1 mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG1 mAb in a 3 fold serial dilution from 1 μg/mL to 6.97×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Mouse IgG1 mAb has highly specific recognition of Mouse IgG1 while no or minimal cross reactivity to Mouse IgG 2a、Mouse IgG 2b、Mouse IgG3、Mouse IgM、Human IgG1、Rat IgG1.

HRP conjugated Rabbit Anti-Mouse IgG1 Recombinant Antibody (84-790)

CATALOG NUMBER: 84-790

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Mouse
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgG2b (Fc) has highly specific recognition of Mouse IgG2b while no or minimal cross reactivity to Mouse IgG1、Mouse IgG2a、Mouse IgG2c、Mouse IgG3、Mouse IgM、Mouse IgE、Mouse IgD、Rat IgG、Human IgG.

HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) Recombinant Antibody (84-795)

CATALOG NUMBER: 84-795

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from Rabbit IgG isotype control cells using HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb at 1:6000 dilution incubated overnight at 4℃.|Lysates/proteins: 100ng μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.

HRP-conjugated Mouse anti-Rabbit IgG Light Chain Recombinant Antibody (84-798)

CATALOG NUMBER: 84-798

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, IP, WB
Conjugate:
HRP
List Size:
100 uL
Western blot analysis of recombinant goat IgG Fab protein using HRP-conjugated Rabbit anti-Goat IgG Fab mAb at 1:500-1:64000 dilution. Blot was incubated with the HRP conjugated antibody in the Blocking buffer (3% non-fat dry milk in TBST) at room temperature for 1 hour before detection using Enhanced chemiluminescence. |Lysates/proteins: Lane 1: molecular weight marker; Lanes 2-9: 100ng goat IgG Fab recombinant Protein per lane. |Detection: ECL Basic Kit. |Exposure time: 90s

HRP-conjugated Rabbit anti-Goat IgG Fab Recombinant Antibody (84-783)

CATALOG NUMBER: 84-783

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
HRP
Species Reactivity:
Goat
List Size:
100 uL
Western blot analysis of recombinant goat IgG Fc Protein using HRP-conjugated Rabbit anti-Goat IgG Fc mAb at 1:500-1:64000 dilution. Blot was incubated with HRP conjugated antibody in the Blocking buffer (3% nonfat dry milk in TBST) at room temperature for 1 hour before detection using Enhanced chemiluminescence. |Lysates/proteins: Lane 1: molecular weight marker; Lanes 2-9: 100ng goat IgG Fc recombinant Protein per lane. |Detection: ECL Basic Kit. |Exposure time: 15s.

HRP-conjugated Rabbit anti-Goat IgG Fc Recombinant Antibody (84-782)

CATALOG NUMBER: 84-782

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
HRP
Species Reactivity:
Goat
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit anti-Human IgA (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit anti-Human IgA (Fc) mAb in a 2 fold serial dilution from 1 μg/mL to 7.8*10^-3 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG4 has highly specific recognition of Human IgA (Fc) while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Human IgM、Human IgE、Human IgD.

HRP-conjugated Rabbit anti-Human IgA (Fc) Recombinant Antibody (84-786)

CATALOG NUMBER: 84-786

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Human IgD mAb measured by ELISA. 25 ng of Human IgD recombinant protein was coated in 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgD mAb in a 2 fold serial dilution from 1 μg/mL to 7.8 pg/mL, incubation was performed at room temperature for 1 hour. EC50 value ( 2.10×10^-2 μg/mL) were calculated using 4 parameter logistic regression.

HRP-conjugated Rabbit anti-Human IgD Recombinant Antibody (84-781)

CATALOG NUMBER: 84-781

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1×10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Anti-Human IgE (Fc) mAb has highly specific recognition of Human IgE while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Human IgM、Mouse IgG、Rat IgG.

HRP-conjugated Rabbit anti-Human IgE Recombinant Antibody (84-787)

CATALOG NUMBER: 84-787

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of vlysates from Human plasma using HRP-conjugated Rabbit anti-Human IgG1 (Fc) mAb at 1:4000-1:32000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30 s.

HRP-conjugated Rabbit anti-Human IgG1 (Fc) Recombinant Antibody (84-780)

CATALOG NUMBER: 84-780

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL