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![Western blot analysis of various lysates using [KD Validated] CDX2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0450_WB_01_81-316.jpg)
![Western blot analysis of various lysates using [KD Validated] CHCHD4 Rabbit mAb at 1:13000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC72750-01_WB_01_83-489.jpg)
![Western blot analysis of lysates from wild type (WT) and Claudin 1 knockdown (KD) Hep G2 cells using [KD Validated] Claudin 1 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC3289_WB_01_83-181.jpg)
![Western blot analysis of various lysates using [KD Validated] COX5A Rabbit mAb at 1:20000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC67578-01_WB_03_83-142.jpg)
![Western blot analysis of lysates from wild type (WT) and CREBBP knockdown (KD) 293T cells using [KD Validated] CREBBP Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC70983-01_WB_01_83-330.jpg)
![Western blot analysis of lysates from wild type (WT) and EGFR knockdown (KD) HeLa cells using [KD Validated] EGFR Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC60697-03_WB_01_82-578.jpg)
![Western blot analysis of various lysates, using [KD Validated] ERK1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC2591_WB_01_81-491.jpg)
![Western blot analysis of various lysates using [KD Validated] ETFDH Rabbit mAb at 1:63000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC70451-01_WB_01_83-346.jpg)
![Western blot analysis of lysates from PC-12 cells using [KD Validated] GM130 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC74579-01_WB_01_83-605.jpg)
![Western blot analysis of lysates from wild type (WT) and KDM6A knockdown (KD) 293T cells using [KD Validated] KDM6A Rabbit mAb at 1:12000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC71730-01_WB_01_83-525.jpg)