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![Western blot analysis of lysates from wild type (WT) and Abhd6 knockout (KO) Mouse brain using [KO Validated] Abhd6 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC74848-01_WB_01_83-575.jpg)
![Western blot analysis of various lysates using [KO Validated] AKT1 Rabbit mAb at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC51585-01_WB_01_82-401.jpg)
![Western blot analysis of various lysates using [KO Validated] AMPKα1 Rabbit mAb at 1:12000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73667-01_WB_01_83-576.jpg)
![Western blot analysis of lysates from wild type (WT) and AMPKα1 knockout (KO) HeLa cells using [KO Validated] AMPKα1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73664-01_WB_01_83-629.jpg)
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![Western blot analysis of various lysates using [KO Validated] Calreticulin Mouse mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/AMC50035-01_WB_01_83-467.jpg)
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