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![Western blot analysis of lysates from CACO-2 cells using [KD Validated] NOX1 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC72716-01_WB_01_83-570.jpg)
![Western blot analysis of lysates from wild type (WT) and Thymidylate synthase knockdown (KD) 293T cells using [KD Validated] Thymidylate synthase Rabbit mAb at 1:5000 dilution. |Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC65474-01_WB_01_82-896.jpg)
![Western blot analysis of extracts from wild type(WT) and NRF2 knockout (KO) HeLa cells, using [KO Validated] NRF2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0806_KO-WB_01_83-713.jpg)
