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![Western blot analysis of lysates from Mouse brain, using [KO Validated] Smad2 Rabbit mAb at 1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC57635-01_WB_01_82-326.jpg)
![Western blot analysis of various lysates, using [KO Validated] Smad3 Rabbit mAb at1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC53858-01_WB_01_82-099.jpg)
![Western blot analysis of lysates from wild type (WT) and Smad3 knockout (KO) 293T cells, using [KO Validated] Smad3 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0490_KO-WB_01_82-639.jpg)
![Western blot analysis of lysates from wild type (WT) and Src knockout (KO) HeLa cells, using [KO Validated] Src Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0378_KO-WB_01_81-385.jpg)
